| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Annexin A2 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000. WB: Approx 35kDa band observed in lysates of cell lines A549, HeLa and NIH3T3 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002858.1). Recommended concentration: 0.5-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ANXA2 |
| Gene ID: | 302 |
| Uniprot ID: | ANXA2_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_001002858.1; NP_004030.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein. |
| Immunogen Sequence: | STVHEILCKLSLEGD |
| Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
| Protein Name | Annexin A2 Annexin Ii Annexin-2 Calpactin I Heavy Chain Calpactin-1 Heavy Chain Chromobindin-8 Lipocortin Ii Placental Anticoagulant Protein Iv Pap-Iv Protein I P36 |
| Database Links | Reactome: R-HSA-445355 Reactome: R-HSA-6798695 Reactome: R-HSA-75205 Reactome: R-HSA-8950505 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix Basement Membrane Melanosome In The Lamina Beneath The Plasma Membrane Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Translocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism |
| Alternative Antibody Names | Anti-Annexin A2 antibody Anti-Annexin Ii antibody Anti-Annexin-2 antibody Anti-Calpactin I Heavy Chain antibody Anti-Calpactin-1 Heavy Chain antibody Anti-Chromobindin-8 antibody Anti-Lipocortin Ii antibody Anti-Placental Anticoagulant Protein Iv antibody Anti-Pap-Iv antibody Anti-Protein I antibody Anti-P36 antibody Anti-ANXA2 antibody Anti-ANX2 antibody Anti-ANX2L4 antibody Anti-CAL1H antibody Anti-LPC2D antibody |
Information sourced from Uniprot.org