Anti-ANILLIN/Scraps antibody (Internal) (STJ70952)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ANILLIN/Scraps (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Applications | Pep-ELISA/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.IF: Strong expression of the protein seen in the nuclei of NIH3T3 and Neuro-2a cells. Recommended concentration: 10ยตg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ANLN |
| Gene ID | 54443 |
| Uniprot ID | ANLN_HUMAN |
| Accession Number | NP_061155.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | TYPDDEKRKNP |
Additional Info
| Post Translational Modifications | Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1. |
| Function | Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration. |
| Protein Name | Anillin |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonCell CortexCell ProjectionBlebMainly Found In The Nucleus During InterphaseColocalizes With Cortical F-Actin Upon Nuclear Envelope Breakdown In Mitosis And Subsequently Concentrates In The Area Of The Prospective Contractile Ring In AnaphaseThis Pattern Persists Until TelophaseWhen The Protein Becomes Concentrated In The Midbody |
| Alternative Antibody Names | Anti-Anillin antibodyAnti-ANLN antibody |
Information sourced from Uniprot.org