• STJ70428 (10µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • STJ70428 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70428 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ANILLIN/Scraps antibody (C-Term) (STJ70428)

SKU:
STJ70428-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human/Mouse/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ANILLIN/Scraps (C-Term) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3-10µg/ml
IF-Strong expression of the protein seen in the nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of MCF7 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ANLN
Gene ID: 54443
Uniprot ID: ANLN_HUMAN
Immunogen Region: C-Term
Accession Number: NP_061155.2; NP_001271230.1; NP_001271231.1
Immunogen Sequence: WQPDACYKPIGKP
Post Translational Modifications Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1.
Function Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.
Protein Name Anillin
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Cell Cortex
Cell Projection
Bleb
Mainly Found In The Nucleus During Interphase
Colocalizes With Cortical F-Actin Upon Nuclear Envelope Breakdown In Mitosis And Subsequently Concentrates In The Area Of The Prospective Contractile Ring In Anaphase
This Pattern Persists Until Telophase
When The Protein Becomes Concentrated In The Midbody
Alternative Antibody Names Anti-Anillin antibody
Anti-ANLN antibody

Information sourced from Uniprot.org

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