![Immunohistochemistry analysis of ANAPC5 in paraffin-embedded human kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102385/391177/STJ29189_2__81161.1713161374.jpg?c=1 "Immunohistochemistry analysis of ANAPC5 in paraffin-embedded human kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of ANAPC5 in paraffin-embedded rat kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102385/391178/STJ29189_3__62893.1713161375.jpg?c=1 "Immunohistochemistry analysis of ANAPC5 in paraffin-embedded rat kidney using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and ANAPC5 knockout (KO) 293T cells, using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102385/391179/STJ29189_4__02772.1713161375.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ANAPC5 knockout (KO) 293T cells, using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102385/391180/STJ29189_5__75357.1713161376.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] ANAPC5 Rabbit polyclonal antibody (STJ29189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
Anti-ANAPC5 antibody (1-250) (STJ29189)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ANAPC5 (1-250) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANAPC5 |
| Gene ID | 51433 |
| Uniprot ID | APC5_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MASVHESLYFNPMMTNGVVH ANVFGIKDWVTPYKIAVLVL LNEMSRTGEGAVSLMERRRL NQLLLPLLQGPDITLSKLYK LIEESCPQLANSVQIRIKLM AEGELKDMEQFFDDLSDSFS GTEPEVHKTSVVGLFLRHMI LAYSKLSFSQVFKLYTALQQ YFQNGEKKTVEDADMELTSR DEGERKMEKEELDVSVREEE VSCSGPLSQKQAEFFLSQQA SLLKNDETKALTPASLQKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2). |
Additional Info
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. |
| Protein Name | Anaphase-Promoting Complex Subunit 5Apc5Cyclosome Subunit 5 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative Antibody Names | Anti-Anaphase-Promoting Complex Subunit 5 antibodyAnti-Apc5 antibodyAnti-Cyclosome Subunit 5 antibodyAnti-ANAPC5 antibodyAnti-APC5 antibody |
Information sourced from Uniprot.org