| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AMID/PRG3 (N-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-4µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
AIFM2 |
| Gene ID: |
84883 |
| Uniprot ID: |
FSP1_HUMAN |
| Immunogen Region: |
N-Term |
| Accession Number: |
NP_116186.1 |
| Immunogen Sequence: |
GSQVSVESGALHC |
| Post Translational Modifications | N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane. |
| Function | A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms. Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis. May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death. Capable of DNA binding in a non-sequence specific way. |
| Protein Name | Ferroptosis Suppressor Protein 1Fsp1Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of DeathAmidP53-Responsive Gene 3 Protein |
| Database Links | Reactome: R-HSA-6803204 |
| Cellular Localisation | Lipid DropletCell MembraneLipid-AnchorCytoplasmMitochondrion MembraneNucleus |
| Alternative Antibody Names | Anti-Ferroptosis Suppressor Protein 1 antibodyAnti-Fsp1 antibodyAnti-Apoptosis-Inducing Factor Homologous Mitochondrion-Associated Inducer Of Death antibodyAnti-Amid antibodyAnti-P53-Responsive Gene 3 Protein antibodyAnti-AIFM2 antibodyAnti-AMID antibodyAnti-PRG3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
