• STJ71657 (0. 1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71657 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71657 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Alpha2-antiplasmin antibody (Internal) (STJ71657)

SKU:
STJ71657-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Alpha2-antiplasmin (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-3-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SERPINF2
Gene ID: 5345
Uniprot ID: A2AP_HUMAN
Immunogen Region: Internal
Accession Number: NP_000925.2; NP_001159393.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_000925.2; NP_001159393.1). Reported variants represent identical protein (NP_000925.2; NP_001159392.1).
Immunogen Sequence: KDFLQSLKGFPRGDK
Post Translational Modifications Proteolytically cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.
Function Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Protein Name Alpha-2-Antiplasmin
Alpha-2-Ap
Alpha-2-Plasmin Inhibitor
Alpha-2-Pi
Serpin F2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-75205
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-2-Antiplasmin antibody
Anti-Alpha-2-Ap antibody
Anti-Alpha-2-Plasmin Inhibitor antibody
Anti-Alpha-2-Pi antibody
Anti-Serpin F2 antibody
Anti-SERPINF2 antibody
Anti-AAP antibody
Anti-PLI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance