| Host: |
Rabbit |
| Applications: |
ELISA/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Rabbit polyclonal anti-Alpha II-Spectrin, cleavage-specific for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000 |
| Storage Instruction: |
Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: |
SPTAN1 |
| Gene ID: |
6709 |
| Uniprot ID: |
SPTN1_HUMAN |
| Specificity: |
The antibody detects a 136 kDa* cleaved fragment corresponding to Alpha II-Spectrin on SDS-PAGE immunoblots of mouse diaphram treated with thapsigargin. The antibody only detects cleaved Alpha II-Spectrin and does not detect full length Alpha II-Spec |
| Immunogen: |
A synthetic peptide (coupled to KLH) corresponding to amino acid residues around the calpain cleavage site, tyrosine 1176 in human Alpha II-spectrin. This sequence has high homology with similar regions in rat and mouse Alpha II-spectrin, but is not |
| Immunogen Sequence: |
Human |
Information sourced from Uniprot.org
