| Short Description : | Rabbit polyclonal ALDH1A3 (1-140) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ALDH1A3 |
| Gene ID: | 220 |
| Uniprot ID: | AL1A3_HUMAN |
| Immunogen Region: | 1-140 |
| Immunogen Sequence: | MATANGAVENGQPDRKPPAL PRPIRNLEVKFTKIFINNEW HESKSGKKFATCNPSTREQI CEVEEGDKPDVDKAVEAAQV AFQRGSPWRRLDALSRGRLL HQLADLVERDRATLAALETM DTGKPFLHAFFIDLEGCIRT |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ALDH1A3 (NP_000684.2). |
| Tissue Specificity | Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney. |
| Function | Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. High specificity for all-trans-retinal as substrate, can also accept acetaldehyde as substrate in vitro but with lower affinity. Required for the biosynthesis of normal levels of retinoate in the embryonic ocular and nasal regions.a critical lipid in the embryonic development of the eye and the nasal region. |
| Protein Name | Retinaldehyde Dehydrogenase 3Raldh-3Raldh3Aldehyde Dehydrogenase 6Aldehyde Dehydrogenase Family 1 Member A3Aldh1a3 |
| Database Links | Reactome: R-HSA-5365859 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Retinaldehyde Dehydrogenase 3 antibodyAnti-Raldh-3 antibodyAnti-Raldh3 antibodyAnti-Aldehyde Dehydrogenase 6 antibodyAnti-Aldehyde Dehydrogenase Family 1 Member A3 antibodyAnti-Aldh1a3 antibodyAnti-ALDH1A3 antibodyAnti-ALDH6 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89137/357586/STJ11100067_1__30225.1713121721.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
