• Western blot analysis of various lysates, using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A-549 using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at dilution of 1:800 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at dilution of 1:800 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of A-549 cells using 3 Mu g ALDH1A1 rabbit monoclonal antibody antibody (STJ11101231). Western blot was performed from the immunoprecipitate using ALDH1A1 rabbit monoclonal antibody (STJ11101231) at a dilition of 1:100000.

Anti-ALDH1A1 antibody (250-350) [S1MR] (STJ11101231)

SKU:
STJ11101231

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ALDH1A1 (250-350) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:10000-1:120000
IF/ICC 1:500-1:1000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALDH1A1
Gene ID: 216
Uniprot ID: AL1A1_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 (NP_000680.2).
Immunogen Sequence: VGKLIKEAAGKSNLKRVTLE LGGKSPCIVLADADLDNAVE FAHHGVFYHQGQCCIAASRI FVEESIYDEFVRRSVERAKK YILGNPLTPGVTQGPQIDKE Q
Tissue Specificity Expressed by erythrocytes (at protein level).
Post Translational Modifications The N-terminus is blocked most probably by acetylation.
Function Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission.
Protein Name Aldehyde Dehydrogenase 1a1
3-Deoxyglucosone Dehydrogenase
Aldh-E1
Alhdii
Aldehyde Dehydrogenase Family 1 Member A1
Aldehyde Dehydrogenase - Cytosolic
Retinal Dehydrogenase 1
Raldh 1
Raldh1
Database Links Reactome: R-HSA-5365859
Reactome: R-HSA-70350
Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Cytosol
Cell Projection
Axon
Alternative Antibody Names Anti-Aldehyde Dehydrogenase 1a1 antibody
Anti-3-Deoxyglucosone Dehydrogenase antibody
Anti-Aldh-E1 antibody
Anti-Alhdii antibody
Anti-Aldehyde Dehydrogenase Family 1 Member A1 antibody
Anti-Aldehyde Dehydrogenase - Cytosolic antibody
Anti-Retinal Dehydrogenase 1 antibody
Anti-Raldh 1 antibody
Anti-Raldh1 antibody
Anti-ALDH1A1 antibody
Anti-ALDC antibody
Anti-ALDH1 antibody
Anti-PUMB1 antibody

Information sourced from Uniprot.org

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