• Immunofluorescence analysis of HepG2 cells using Albumin Rabbit polyclonal antibody (STJ22575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using Albumin Rabbit polyclonal antibody (STJ22575) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22575)

SKU:
STJ22575

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Albumin (25-609) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALB
Gene ID: 213
Uniprot ID: ALBU_HUMAN
Immunogen Region: 25-609
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-609 of human Albumin (NP_000468.1).
Immunogen Sequence: DAHKSEVAHRFKDLGEENFK ALVLIAFAQYLQQCPFEDHV KLVNEVTEFAKTCVADESAE NCDKSLHTLFGDKLCTVATL RETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEV DVMCTAFHDNEETFLKKYLY EIARRHPYFYAPELLFFAKR YKAAFTECCQAADKAACLLP KLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLS QRFPKAEFAEVSKLVTDLT
Tissue Specificity Plasma.
Post Translational Modifications Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylated by FAM20C in the extracellular medium. Acetylated on Lys-223 by acetylsalicylic acid.
Function Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
Protein Name Albumin
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-159418
Reactome: R-HSA-189451
Reactome: R-HSA-189483
Reactome: R-HSA-2168880
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Reactome: R-HSA-8964058
Reactome: R-HSA-9707564
Reactome: R-HSA-9749641
Reactome: R-HSA-9757110
Reactome: R-HSA-9793528
Cellular Localisation Secreted
Alternative Antibody Names Anti-Albumin antibody
Anti-ALB antibody
Anti-GIG20 antibody
Anti-GIG42 antibody
Anti-PRO0903 antibody
Anti-PRO1708 antibody
Anti-PRO2044 antibody
Anti-PRO2619 antibody
Anti-PRO2675 antibody
Anti-UNQ696 antibody
Anti-PRO1341 antibody

Information sourced from Uniprot.org

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