| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AKT3 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-1-3µg/mlIHC-5µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
AKT3 |
| Gene ID: |
10000 |
| Uniprot ID: |
AKT3_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_005456.1; NP_859029.1 |
| Specificity: |
This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1). |
| Immunogen Sequence: |
CSPTSQIDNIGEEEM |
| Post Translational Modifications | Phosphorylation on Thr-305 and Ser-472 is required for full activity. Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1. |
| Function | AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
| Protein Name | Rac-Gamma Serine/Threonine-Protein KinaseProtein Kinase Akt-3Protein Kinase B GammaPkb GammaRac-Pk-GammaStk-2 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1257604Reactome: R-HSA-1358803Reactome: R-HSA-198323Reactome: R-HSA-198693Reactome: R-HSA-199418Reactome: R-HSA-211163Reactome: R-HSA-389357Reactome: R-HSA-389513Reactome: R-HSA-392451Reactome: R-HSA-5218920Reactome: R-HSA-5628897Reactome: R-HSA-5674400Reactome: R-HSA-6804757Reactome: R-HSA-6804758Reactome: R-HSA-6804759Reactome: R-HSA-69202Reactome: R-HSA-69656Reactome: R-HSA-8876198Reactome: R-HSA-8941332Reactome: R-HSA-8948751Reactome: R-HSA-9607240Reactome: R-HSA-9614399Reactome: R-HSA-9634638Reactome: R-HSA-9755511Reactome: R-HSA-9755779 |
| Cellular Localisation | NucleusCytoplasmMembranePeripheral Membrane ProteinMembrane-Associated After Cell Stimulation Leading To Its Translocation |
| Alternative Antibody Names | Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibodyAnti-Protein Kinase Akt-3 antibodyAnti-Protein Kinase B Gamma antibodyAnti-Pkb Gamma antibodyAnti-Rac-Pk-Gamma antibodyAnti-Stk-2 antibodyAnti-AKT3 antibodyAnti-PKBG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
