Western blot analysis of various lysates using AKR1B10 Rabbit polyclonal antibody (STJ110133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AKR1B10 antibody (1-316) (STJ110133)

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STJ110133
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1B10
Gene ID: 57016
Uniprot ID: AK1BA_HUMAN
Immunogen Region: 1-316
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B10 (NP_064695.3).
Immunogen Sequence: MATFVELSTKAKMPIVGLGT WKSPLGKVKEAVKVAIDAGY RHIDCAYVYQNEHEVGEAIQ EKIQEKAVKREDLFIVSKLW PTFFERPLVRKAFEKTLKDL KLSYLDVYLIHWPQGFKSGD DLFPKDDKGNAIGGKATFLD AWEAMEELVDEGLVKALGVS NFSHFQIEKLLNKPGLKYKP VTNQVECHPYLTQEKLIQYC HSKGITVTAYSPLGSPDRPW AKPEDPSLLEDPKIKEIAA
Tissue Specificity Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose.
Protein Name Aldo-Keto Reductase Family 1 Member B10
Arl-1
Aldose Reductase-Like
Aldose Reductase-Related Protein
Arp
Harp
Small Intestine Reductase
Si Reductase
Database Links Reactome: R-HSA-975634
Cellular Localisation Lysosome
Secreted
Secreted Through A Lysosome-Mediated Non-Classical Pathway
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member B10 antibody
Anti-Arl-1 antibody
Anti-Aldose Reductase-Like antibody
Anti-Aldose Reductase-Related Protein antibody
Anti-Arp antibody
Anti-Harp antibody
Anti-Small Intestine Reductase antibody
Anti-Si Reductase antibody
Anti-AKR1B10 antibody
Anti-AKR1B11 antibody

Information sourced from Uniprot.org

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