• Western blot analysis of the lysates from HUVECcells using AKR1B1 antibody.
  • Western blot analysis of lysates from HUVEC cells, using AKR1B1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of HuvEc cells using Aldose Reductase Polyclonal Antibody
  • Western blot analysis of various cells using Aldose Reductase Polyclonal Antibody

Anti-AKR1B1 antibody (241-290 aa) (STJ91559)

SKU:
STJ91559

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Aldo-keto reductase family 1 member B1 (241-290 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1B1
Gene ID: 231
Uniprot ID: ALDR_HUMAN
Immunogen Region: 241-290 aa
Specificity: Aldose Reductase Polyclonal Antibody detects endogenous levels of Aldose Reductase protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AKR1B1 at the amino acid range 241-290
Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).
Protein Name Aldo-Keto Reductase Family 1 Member B1
Aldehyde Reductase
Aldose Reductase
Ar
Database Links Reactome: R-HSA-196108
Reactome: R-HSA-5652227
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member B1 antibody
Anti-Aldehyde Reductase antibody
Anti-Aldose Reductase antibody
Anti-Ar antibody
Anti-AKR1B1 antibody
Anti-ALDR1 antibody
Anti-ALR2 antibody

Information sourced from Uniprot.org

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