• Western blot analysis of extracts of various cell lines, using AKR1A1 antibody (STJ22566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AKR1A1 antibody (1-325) (STJ22566)

SKU:
STJ22566

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1A1
Gene ID: 10327
Uniprot ID: AK1A1_HUMAN
Immunogen Region: 1-325
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1).
Immunogen Sequence: MAASCVLLHTGQKMPLIGLG TWKSEPGQVKAAVKYALSVG YRHIDCAAIYGNEPEIGEAL KEDVGPGKAVPREELFVTSK LWNTKHHPEDVEPALRKTLA DLQLEYLDLYLMHWPYAFER GDNPFPKNADGTICYDSTHY KETWKALEALVAKGLVQALG LSNFNSRQIDDILSVASVRP AVLQVECHPYLAQNELIAHC QARGLEVTAYSPLGSSDRAW RDPDEPVLLEEPVVLALAE
Tissue Specificity Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung.
Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. S-nitroso-CoA reductase activity is involved in reprogramming intermediary metabolism in renal proximal tubules, notably by inhibiting protein S-nitrosylation of isoform 2 of PKM (PKM2). Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids.
Protein Name Aldo-Keto Reductase Family 1 Member A1
Alcohol Dehydrogenase Nadp(+
Aldehyde Reductase
Glucuronate Reductase
Glucuronolactone Reductase
S-Nitroso-Coa Reductase
Scorr
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-5661270
Cellular Localisation Cytoplasm
Cytosol
Apical Cell Membrane
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member A1 antibody
Anti-Alcohol Dehydrogenase Nadp(+ antibody
Anti-Aldehyde Reductase antibody
Anti-Glucuronate Reductase antibody
Anti-Glucuronolactone Reductase antibody
Anti-S-Nitroso-Coa Reductase antibody
Anti-Scorr antibody
Anti-AKR1A1 antibody
Anti-ALDR1 antibody
Anti-ALR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance