| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AKAP9/AKAP450/CG-NAP (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-1-3µg/mlIF-Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. 10µg/mlELISA-antibody detection limit dilution 1:64000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
AKAP9 |
| Gene ID: |
10142 |
| Uniprot ID: |
AKAP9_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_005742.4; NP_671714.1 |
| Specificity: |
This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized. |
| Immunogen Sequence: |
SGSTTQFHAGMR |
| Function | Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus. Required for microtubule nucleation at the cis-side of the Golgi apparatus. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery. Isoform 4: Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. |
| Protein Name | A-Kinase Anchor Protein 9Akap-9A-Kinase Anchor Protein 350 KdaAkap 350Hgakap 350A-Kinase Anchor Protein 450 KdaAkap 450Akap 120-Like ProteinCentrosome- And Golgi-Localized Pkn-Associated ProteinCg-NapProtein HyperionProtein Kinase A-Anchoring Protein 9Prka9Protein Yotiao |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5576890Reactome: R-HSA-5576893Reactome: R-HSA-5620912Reactome: R-HSA-6802952Reactome: R-HSA-8854518 |
| Cellular Localisation | Golgi ApparatusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCytoplasmic In Parietal CellsRecruited To The Golgi Apparatus By Gm130/Golga2Localization At The Centrosome Versus Golgi Apparatus May Be Cell Line-DependentIn Skbr3 And Hek293f CellsExclusively Located At The CentrosomeIn HelaMda-Mb231 And Rpe-1 CellsDetected At The Golgi ApparatusIn Sk-Br-3 CellsRecruited To The Centrosome In The Presence Of Cdk5rap2 |
| Alternative Antibody Names | Anti-A-Kinase Anchor Protein 9 antibodyAnti-Akap-9 antibodyAnti-A-Kinase Anchor Protein 350 Kda antibodyAnti-Akap 350 antibodyAnti-Hgakap 350 antibodyAnti-A-Kinase Anchor Protein 450 Kda antibodyAnti-Akap 450 antibodyAnti-Akap 120-Like Protein antibodyAnti-Centrosome- And Golgi-Localized Pkn-Associated Protein antibodyAnti-Cg-Nap antibodyAnti-Protein Hyperion antibodyAnti-Protein Kinase A-Anchoring Protein 9 antibodyAnti-Prka9 antibodyAnti-Protein Yotiao antibodyAnti-AKAP9 antibodyAnti-AKAP350 antibodyAnti-AKAP450 antibodyAnti-KIAA0803 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
