| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AKAP5 |
| Gene ID: |
9495 |
| Uniprot ID: |
AKAP5_HUMAN |
| Immunogen Region: |
1-270 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3). |
| Immunogen Sequence: |
METTISEIHVENKDEKRSAE GSPGAERQKEKASMLCFKRR KKAAKALKPKAGSEAADVAR KCPQEAGASDQPEPTRGAWA SLKRLVTRRKRSESSKQQKP LEGEMQPAINAEDADLSKKK AKSRLKIPCIKFPRGPKRSN HSKIIEDSDCSIKVQEEAEI LDIQTQTPLNDQATKAKSTQ DLSEGISRKDGDEVCESNVS NSTTSGEKVISVELGLDNGH SAIQTGTLILEEIETIKEK |
| Tissue Specificity | Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary. |
| Post Translational Modifications | Palmitoylated. Palmitoylation at Cys-36 and Cys-129 play a key role in the targeting of AKAP5 to lipid rafts. Palmitoylation by ZDHHC2 is required for AKAP5 function in LTP-stimulated recycling endosome exocytosis. |
| Function | Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses. In hippocampal pyramidal neurons, recruits KCNK2/TREK-1 channel at postsynaptic dense bodies microdomains and converts it to a leak channel no longer sensitive to stimulation by arachidonic acid, acidic pH or mechanical stress, nor inhibited by Gq-coupled receptors but still under the negative control of Gs-coupled receptors. Associates with ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where it recruits NFATC2/NFAT1 and couples store-operated Ca(2+) influx to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses. |
| Protein Name | A-Kinase Anchor Protein 5Akap-5A-Kinase Anchor Protein 79 KdaAkap 79H21Camp-Dependent Protein Kinase Regulatory Subunit Ii High Affinity-Binding Protein |
| Database Links | Reactome: R-HSA-381676Reactome: R-HSA-399719Reactome: R-HSA-9010642 |
| Cellular Localisation | Postsynaptic Recycling Endosome MembraneLipid-AnchorCell ProjectionDendritePostsynaptic Cell MembraneAssociates With Lipid Rafts |
| Alternative Antibody Names | Anti-A-Kinase Anchor Protein 5 antibodyAnti-Akap-5 antibodyAnti-A-Kinase Anchor Protein 79 Kda antibodyAnti-Akap 79 antibodyAnti-H21 antibodyAnti-Camp-Dependent Protein Kinase Regulatory Subunit Ii High Affinity-Binding Protein antibodyAnti-AKAP5 antibodyAnti-AKAP79 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
