| Short Description : | Rabbit polyclonal AK3 (1-227) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AK3 |
| Gene ID: | 50808 |
| Uniprot ID: | KAD3_HUMAN |
| Immunogen Region: | 1-227 |
| Immunogen Sequence: | MGASARLLRAVIMGAPGSGK GTVSSRITTHFELKHLSSGD LLRDNMLRGTEIGVLAKAFI DQGKLIPDDVMTRLALHELK NLTQYSWLLDGFPRTLPQAE ALDRAYQIDTVINLNVPFEV IKQRLTARWIHPASGRVYNI EFNPPKTVGIDDLTGEPLIQ REDDKPETVIKRLKAYEDQT KPVLEYYQKKGVLETFSGTE TNKIWPYVYAFLQTKVPQRS QKASVTP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human AK3 (NP_057366.2). |
| Tissue Specificity | Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung. |
| Function | Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity. Could also use ITP as phosphate donor. Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable). |
| Protein Name | Gtp -Amp Phosphotransferase Ak3 - MitochondrialAdenylate Kinase 3Adenylate Kinase 3 Alpha-Like 1Adenylate Kinase Isozyme 3 |
| Database Links | Reactome: R-HSA-983231 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Gtp -Amp Phosphotransferase Ak3 - Mitochondrial antibodyAnti-Adenylate Kinase 3 antibodyAnti-Adenylate Kinase 3 Alpha-Like 1 antibodyAnti-Adenylate Kinase Isozyme 3 antibodyAnti-AK3 antibodyAnti-AK3L1 antibodyAnti-AK6 antibodyAnti-AKL3L antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89770/358242/STJ11100835_1__03090.1713122575.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
