Western blot analysis of extracts of various cell lines, using AIF1/AIF1/IBA1 rabbit monoclonal antibody (STJ11103127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AIF1/IBA1 antibody (1-100) [S7MR] (STJ11103127)

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STJ11103127
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:4000
IHC-P:1:200-1:2000
IF/ICC:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Aif1
Gene ID: 11629
Uniprot ID: AIF1_MOUSE
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse AIF1/IBA1 (O70200).
Immunogen Sequence: MSQSRDLQGGKAFGLLKAQQ EERLEGINKQFLDDPKYSND EDLPSKLEAFKVKYMEFDLN GNGDIDIMSLKRMLEKLGVP KTHLELKRLIREVSSGSEET
Tissue Specificity Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages.
Post Translational Modifications Phosphorylated on serine residues.
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name Allograft Inflammatory Factor 1
Aif-1
Ionized Calcium-Binding Adapter Molecule 1
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Phagocytic Cup
Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative Antibody Names Anti-Allograft Inflammatory Factor 1 antibody
Anti-Aif-1 antibody
Anti-Ionized Calcium-Binding Adapter Molecule 1 antibody
Anti-Aif1 antibody
Anti-Iba1 antibody

Information sourced from Uniprot.org

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