• Western blot analysis of extracts of mouse skeletal muscle, using AGRP antibody (STJ11100945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse adrenal gland using AGRP Rabbit polyclonal antibody (STJ11100945) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse adrenal gland using AGRP rabbit polyclonal antibody (STJ11100945) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse skeletal muscle, using AGRP antibody (STJ11100945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AGRP antibody (21-132) (STJ11100945)

SKU:
STJ11100945

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGRP (21-132) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGRP
Gene ID: 181
Uniprot ID: AGRP_HUMAN
Immunogen Region: 21-132
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-132 of human AGRP (NP_001129.1).
Immunogen Sequence: AQMGLAPMEGIRRPDQALLP ELPGLGLRAPLKKTTAEQAE EDLLQEAQALAEVLDLQDRE PRSSRRCVRLHESCLGQQVP CCDPCATCYCRFFNAFCYCR KLGTAMNPCSRT
Tissue Specificity Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
Function Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
Protein Name Agouti-Related Protein
Database Links Reactome: R-HSA-9615017
Cellular Localisation Secreted
Golgi Apparatus Lumen
Alternative Antibody Names Anti-Agouti-Related Protein antibody
Anti-AGRP antibody
Anti-AGRT antibody
Anti-ART antibody

Information sourced from Uniprot.org

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