| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-AGO1 (1-170) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AGO1 |
| Gene ID: | 26523 |
| Uniprot ID: | AGO1_HUMAN |
| Immunogen Region: | 1-170 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human AGO1 (NP_036331.1). |
| Immunogen Sequence: | MEAGPSGAAAGAYLPPLQQV FQAPRRPGIGTVGKPIKLLA NYFEVDIPKIDVYHYEVDIK PDKCPRRVNREVVEYMVQHF KPQIFGDRKPVYDGKKNIYT VTALPIGNERVDFEVTIPGE GKDRIFKVSIKWLAIVSWRM LHEALVSGQIPVPLESVQAL DVAMRHLASM |
| Post Translational Modifications | Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO1 when it is engaged with a TDMD target. |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). |
| Protein Name | Protein Argonaute-1Argonaute1Hago1Argonaute Risc Catalytic Component 1Eukaryotic Translation Initiation Factor 2c 1Eif-2c 1Eif2c 1Putative Rna-Binding Protein Q99 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-203927Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-4086398Reactome: R-HSA-426486Reactome: R-HSA-426496Reactome: R-HSA-5578749Reactome: R-HSA-5628897Reactome: R-HSA-5687128Reactome: R-HSA-8853884Reactome: R-HSA-8934593Reactome: R-HSA-8936459Reactome: R-HSA-8943723Reactome: R-HSA-8948700Reactome: R-HSA-8986944Reactome: R-HSA-9018519Reactome: R-HSA-9022692Reactome: R-HSA-9029569Reactome: R-HSA-9759811Reactome: R-HSA-9768778 |
| Cellular Localisation | CytoplasmP-Body |
| Alternative Antibody Names | Anti-Protein Argonaute-1 antibodyAnti-Argonaute1 antibodyAnti-Hago1 antibodyAnti-Argonaute Risc Catalytic Component 1 antibodyAnti-Eukaryotic Translation Initiation Factor 2c 1 antibodyAnti-Eif-2c 1 antibodyAnti-Eif2c 1 antibodyAnti-Putative Rna-Binding Protein Q99 antibodyAnti-AGO1 antibodyAnti-EIF2C1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
