| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | ADSL |
| Gene ID: | 158 |
| Uniprot ID: | PUR8_HUMAN |
| Immunogen Region: | 1-484 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-484 of human ADSL (NP_000017.1). |
| Immunogen Sequence: | MAAGGDHGSPDSYRSPLASR YASPEMCFVFSDRYKFRTWR QLWLWLAEAEQTLGLPITDE QIQEMKSNLENIDFKMAAEE EKRLRHDVMAHVHTFGHCCP KAAGIIHLGATSCYVGDNTD LIILRNALDLLLPKLARVIS RLADFAKERASLPTLGFTHF QPAQLTTVGKRCCLWIQDLC MDLQNLKRVRDDLRFRGVKG TTGTQASFLQLFEGDDHKVE QLDKMVTEKAGFKRAFIIT |
| Tissue Specificity | Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1. |
| Function | Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate. |
| Protein Name | Adenylosuccinate LyaseAdslAslAdenylosuccinaseAsase |
| Database Links | Reactome: R-HSA-73817 |
| Alternative Antibody Names | Anti-Adenylosuccinate Lyase antibodyAnti-Adsl antibodyAnti-Asl antibodyAnti-Adenylosuccinase antibodyAnti-Asase antibodyAnti-ADSL antibodyAnti-AMPS antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")