Western blot analysis of various lysates using ADRA2B Rabbit pAb (STJ111276) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ADRA2B antibody (1-100) (STJ111276)

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STJ111276
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Short Description :Rabbit polyclonal ADRA2B (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:ADRA2B
Gene ID:151
Uniprot ID:ADA2B_HUMAN
Immunogen Region:1-100
Immunogen Sequence:MDHQDPYSVQATAAIAAAIT FLILFTIFGNALVILAVLTS RSLRAPQNLFLVSLAAADIL VATLIIPFSLANELLGYWYF RRTWCEVYLALDVLFCTSSI
Specificity:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRA2B (NP_000673.2).
Function Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol.
Protein Name Alpha-2b Adrenergic Receptor
Alpha-2 Adrenergic Receptor Subtype C2
Alpha-2b Adrenoreceptor
Alpha-2b Adrenoceptor
Alpha-2bar
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-392023
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Interaction With Rab26
Gga1
Gga2 And Gga3 Mediates Transport From The Golgi To The Cell Membrane
Alternative Antibody Names Anti-Alpha-2b Adrenergic Receptor antibody
Anti-Alpha-2 Adrenergic Receptor Subtype C2 antibody
Anti-Alpha-2b Adrenoreceptor antibody
Anti-Alpha-2b Adrenoceptor antibody
Anti-Alpha-2bar antibody
Anti-ADRA2B antibody
Anti-ADRA2L1 antibody
Anti-ADRA2RL1 antibody

Information sourced from Uniprot.org

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