Western blot analysis of extracts of various cell lines, using ADRA1B antibody (STJ110182) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADRA1B antibody (500-600) (STJ110182)

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STJ110182
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADRA1B
Gene ID: 147
Uniprot ID: ADA1B_HUMAN
Immunogen Region: 500-600
Specificity: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ADRA1B (P35368).
Immunogen Sequence: ADVANGQPGFKSNMPLAPGQ F
Function This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Protein Name Alpha-1b Adrenergic Receptor
Alpha-1b Adrenoreceptor
Alpha-1b Adrenoceptor
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cytoplasm
Membrane
Caveola
Location At The Nuclear Membrane Facilitates Heterooligomerization And Regulates Erk-Mediated Signaling In Cardiac Myocytes
Signaling In Cardiac Myocytes
Colocalizes With Gnaq
Plcb1 As Well As Lap2 At The Nuclear Membrane Of Cardiac Myocytes
Alternative Antibody Names Anti-Alpha-1b Adrenergic Receptor antibody
Anti-Alpha-1b Adrenoreceptor antibody
Anti-Alpha-1b Adrenoceptor antibody
Anti-ADRA1B antibody

Information sourced from Uniprot.org

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