• Western blot analysis of extracts of various cell lines, using ADRA1B antibody (STJ110182) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ADRA1B antibody (STJ110182) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ADRA1B antibody (500-600) (STJ110182)

SKU:
STJ110182

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADRA1B (500-600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADRA1B
Gene ID: 147
Uniprot ID: ADA1B_HUMAN
Immunogen Region: 500-600
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ADRA1B (P35368).
Immunogen Sequence: ADVANGQPGFKSNMPLAPGQ F
Function This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Protein Name Alpha-1b Adrenergic Receptor
Alpha-1b Adrenoreceptor
Alpha-1b Adrenoceptor
Database Links Reactome: R-HSA-390696
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Cellular Localisation Nucleus Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cytoplasm
Membrane
Caveola
Location At The Nuclear Membrane Facilitates Heterooligomerization And Regulates Erk-Mediated Signaling In Cardiac Myocytes
Signaling In Cardiac Myocytes
Colocalizes With Gnaq
Plcb1 As Well As Lap2 At The Nuclear Membrane Of Cardiac Myocytes
Alternative Antibody Names Anti-Alpha-1b Adrenergic Receptor antibody
Anti-Alpha-1b Adrenoreceptor antibody
Anti-Alpha-1b Adrenoceptor antibody
Anti-ADRA1B antibody

Information sourced from Uniprot.org

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