| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ADK |
| Gene ID: | 132 |
| Uniprot ID: | ADK_HUMAN |
| Immunogen Region: | 200-345 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-345 of human ADK (NP_001114.2). |
| Immunogen Sequence: | PFISQFYKESLMKVMPYVDI LFGNETEAATFAREQGFETK DIKEIAKKTQALPKMNSKRQ RIVIFTQGRDDTIMATESEV TAFAVLDQDQKEIIDTNGAG DAFVGGFLSQLVSDKPLTEC IRAGHYAASIIIRRTGCTFP EKPDFH |
| Tissue Specificity | Widely expressed. Highest level in placenta, liver, muscle and kidney. |
| Function | Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. |
| Protein Name | Adenosine KinaseAkAdenosine 5'-Phosphotransferase |
| Database Links | Reactome: R-HSA-74217Reactome: R-HSA-9755088 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Adenosine Kinase antibodyAnti-Ak antibodyAnti-Adenosine 5'-Phosphotransferase antibodyAnti-ADK antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ADK knockout (KO) HeLa cells, using [KO Validated] ADK Rabbit polyclonal antibody (STJ11100868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89802/358269/STJ11100868_1__38447.1713122611.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ADK knockout (KO) HeLa cells, using [KO Validated] ADK Rabbit polyclonal antibody (STJ11100868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
