• Western blot analysis of extracts of various cell lines, using ADH7 antibody (STJ110181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using ADH7 antibody (STJ110181) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using ADH7 antibody (STJ110181) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ADH7 antibody (STJ110181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADH7 antibody (1-386) (STJ110181)

SKU:
STJ110181

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADH7 (1-386) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADH7
Gene ID: 131
Uniprot ID: ADH7_HUMAN
Immunogen Region: 1-386
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-386 of human ADH7 (NP_000664.2).
Immunogen Sequence: MFAEIQIQDKDRMGTAGKVI KCKAAVLWEQKQPFSIEEIE VAPPKTKEVRIKILATGICR TDDHVIKGTMVSKFPVIVGH EATGIVESIGEGVTTVKPGD KVIPLFLPQCRECNACRNPD GNLCIRSDITGRGVLADGTT RFTCKGKPVHHFMNTSTFTE YTVVDESSVAKIDDAAPPEK VCLIGCGFSTGYGAAVKTGK VKPGSTCVVFGLGGVGLSVI MGCKSAGASRIIGIDLNKD
Tissue Specificity Preferentially expressed in stomach.
Function Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives. Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives. Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal. Catalyzes in the oxidative direction with higher efficiency. Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation.
Protein Name All-Trans-Retinol Dehydrogenase Nad(+ Adh7
Alcohol Dehydrogenase Class 4 Mu/Sigma Chain
Alcohol Dehydrogenase Class Iv Mu/Sigma Chain
Gastric Alcohol Dehydrogenase
Omega-Hydroxydecanoate Dehydrogenase Adh7
Retinol Dehydrogenase
Database Links Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-All-Trans-Retinol Dehydrogenase Nad(+ Adh7 antibody
Anti-Alcohol Dehydrogenase Class 4 Mu/Sigma Chain antibody
Anti-Alcohol Dehydrogenase Class Iv Mu/Sigma Chain antibody
Anti-Gastric Alcohol Dehydrogenase antibody
Anti-Omega-Hydroxydecanoate Dehydrogenase Adh7 antibody
Anti-Retinol Dehydrogenase antibody
Anti-ADH7 antibody

Information sourced from Uniprot.org

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