• Western blot analysis of extracts of various cell lines, using ADH5/GSNOR antibody (STJ115420) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using ADH5/GSNOR rabbit polyclonal antibody (STJ115420) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using ADH5/GSNOR rabbit polyclonal antibody (STJ115420) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR rabbit polyclonal antibody (STJ115420) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ADH5/GSNOR rabbit polyclonal antibody (STJ115420) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ADH5 antibody (1-374) (STJ115420)

SKU:
STJ115420

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADH5
Gene ID: 128
Uniprot ID: ADHX_HUMAN
Immunogen Region: 1-374
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3).
Immunogen Sequence: MANEVIKCKAAVAWEAGKPL SIEEIEVAPPKAHEVRIKII ATAVCHTDAYTLSGADPEGC FPVILGHEGAGIVESVGEGV TKLKAGDTVIPLYIPQCGEC KFCLNPKTNLCQKIRVTQGK GLMPDGTSRFTCKGKTILHY MGTSTFSEYTVVADISVAKI DPLAPLDKVCLLGCGISTGY GAAVNTAKLEPGSVCAVFGL GGVGLAVIMGCKVAGASRII GVDINKDKFARAKEFGATE
Function Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation.
Protein Name Alcohol Dehydrogenase Class-3
Alcohol Dehydrogenase 5
Alcohol Dehydrogenase Class Chi Chain
Alcohol Dehydrogenase Class-Iii
Glutathione-Dependent Formaldehyde Dehydrogenase
Faldh
Fdh
Gsh-Fdh
S-(Hydroxymethylglutathione Dehydrogenase
Database Links Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Alcohol Dehydrogenase Class-3 antibody
Anti-Alcohol Dehydrogenase 5 antibody
Anti-Alcohol Dehydrogenase Class Chi Chain antibody
Anti-Alcohol Dehydrogenase Class-Iii antibody
Anti-Glutathione-Dependent Formaldehyde Dehydrogenase antibody
Anti-Faldh antibody
Anti-Fdh antibody
Anti-Gsh-Fdh antibody
Anti-S-(Hydroxymethylglutathione Dehydrogenase antibody
Anti-ADH5 antibody
Anti-ADHX antibody
Anti-FDH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance