• Immunofluorescence analysis of HepG2 cells using Adenylate kinase 4 Rabbit monoclonal antibody (STJ11103485) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Adenylate kinase 4 Rabbit monoclonal antibody (STJ11103485) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Adenylate kinase 4 antibody (STJ11103485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Adenylate kinase 4 antibody (1-100) [S5MR] (STJ11103485)

SKU:
STJ11103485

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Adenylate kinase 4 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK4
Gene ID: 205
Uniprot ID: KAD4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Adenylate kinase 4 (P27144).
Immunogen Sequence: MASKLLRAVILGPPGSGKGT VCQRIAQNFGLQHLSSGHFL RENIKASTEVGEMAKQYIEK SLLVPDHVITRLMMSELENR RGQHWLLDGFPRTLGQAEAL
Tissue Specificity Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain.
Function Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity. Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK. Plays a protective role in the cellular response to oxidative stress.
Protein Name Adenylate Kinase 4 - Mitochondrial
Ak 4
Adenylate Kinase 3-Like
Gtp -Amp Phosphotransferase Ak4
Database Links Reactome: R-HSA-499943
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Adenylate Kinase 4 - Mitochondrial antibody
Anti-Ak 4 antibody
Anti-Adenylate Kinase 3-Like antibody
Anti-Gtp -Amp Phosphotransferase Ak4 antibody
Anti-AK4 antibody
Anti-AK3 antibody
Anti-AK3L1 antibody

Information sourced from Uniprot.org

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