• Immunofluorescence analysis of SH-SY5Y cells using ADCYAP1R1 Rabbit polyclonal antibody (STJ22514) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ADCYAP1R1 Rabbit polyclonal antibody (STJ22514) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ADCYAP1R1 Rabbit polyclonal antibody (STJ22514) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from U-87MG cells, using ADCYAP1R1 Rabbit polyclonal antibody (STJ22514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from Mouse heart, using ADCYAP1R1 Rabbit polyclonal antibody (STJ22514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ADCYAP1R1 antibody (25-140) (STJ22514)

SKU:
STJ22514

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADCYAP1R1 (25-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADCYAP1R1
Gene ID: 117
Uniprot ID: PACR_HUMAN
Immunogen Region: 25-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-140 of human ADCYAP1R1 (NP_001109.2).
Immunogen Sequence: CIFKKEQAMCLEKIQRANEL MGFNDSSPGCPGMWDNITCW KPAHVGEMVLVSCPELFRIF NPDQVWETETIGESDFGDSN SLDLSDMGVVSRNCTEDGWS EPFPHYFDACGFDEYE
Tissue Specificity Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.
Function This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
Protein Name Pituitary Adenylate Cyclase-Activating Polypeptide Type I Receptor
Pacap Type I Receptor
Pacap-R-1
Pacap-R1
Database Links Reactome: R-HSA-187024
Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Pituitary Adenylate Cyclase-Activating Polypeptide Type I Receptor antibody
Anti-Pacap Type I Receptor antibody
Anti-Pacap-R-1 antibody
Anti-Pacap-R1 antibody
Anti-ADCYAP1R1 antibody

Information sourced from Uniprot.org

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