Anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 antibody (C-Term) (STJA0040888)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenThe C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST.
General Information
| Short Description | Rabbit polyclonal anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (C-Term) for use in ICC and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 0.73611111111111116IP 3 µg/reaction |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at |
Target Information
| Gene Symbol | APPL1 |
| Gene ID | 26060 |
| Uniprot ID | DP13A_HUMAN |
| Immunogen | The C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST. |
| Immunogen Region | C-Term |
| Immunogen Sequence | Human |
| Specificity | This antiserum is known to recognize both mouse and human APPL1. |
Additional Info
| Tissue Specificity | High levels in heart, ovary, pancreas and skeletal muscle. |
| Post Translational Modifications | Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL. |
| Function | Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages. Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. |
| Protein Name | Dcc-Interacting Protein 13-AlphaDip13-AlphaAdapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 1 |
| Database Links | Reactome: R-HSA-418889 |
| Cellular Localisation | Early Endosome MembranePeripheral Membrane ProteinNucleusCytoplasmEndosomeCell ProjectionRuffleCytoplasmic VesiclePhagosomeEarly Endosomal Membrane-Bound And NuclearTranslocated Into The Nucleus Upon Release From Endosomal Membranes Following Internalization Of Egf |
| Alternative Antibody Names | Anti-Dcc-Interacting Protein 13-Alpha antibodyAnti-Dip13-Alpha antibodyAnti-Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 1 antibodyAnti-APPL1 antibodyAnti-APPL antibodyAnti-DIP13A antibodyAnti-KIAA1428 antibody |
Information sourced from Uniprot.org