• Western blot analysis of extracts of 293T cells, using ADAP2 antibody (STJ115690) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ADAP2 antibody (1-381) (STJ115690)

SKU:
STJ115690

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAP2 (1-381) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAP2
Gene ID: 55803
Uniprot ID: ADAP2_HUMAN
Immunogen Region: 1-381
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-381 of human ADAP2 (NP_060874.1).
Immunogen Sequence: MGDRERNKKRLLELLRAPDT GNAHCADCGAADPDWASYKL GIFICLNCCGVHRNFPDISR VKSVRLDFWDDSIVEFMIHN GNLRVKAKFEARVPAFYYIP QANDCLVLKEQWIRAKYERR EFMADGETISLPGNREGFLW KRGRDNSQFLRRKFVLLARE GLLKYFTKEQGKSPKAVISI KDLNATFQTEKIGHPHGLQI TYRRDGHTRNLFVYHESGKE IVDWFNALRAARLQYLKMA
Tissue Specificity Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow.
Function GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity.
Protein Name Arf-Gap With Dual Ph Domain-Containing Protein 2
Centaurin-Alpha-2
Cnt-A2
Cellular Localisation Cytoplasm
Cell Membrane
Constitutively Associated With The Plasma Membrane
Excluded From The Nucleus
Alternative Antibody Names Anti-Arf-Gap With Dual Ph Domain-Containing Protein 2 antibody
Anti-Centaurin-Alpha-2 antibody
Anti-Cnt-A2 antibody
Anti-ADAP2 antibody
Anti-CENTA2 antibody

Information sourced from Uniprot.org

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