| Host: |
Rabbit |
| Applications: |
WB/ICC/IHC |
| Reactivity: |
Chicken/Human/Mouse/Rat/Xenopus |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Arp3 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHC 1:100ICC 1:250 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ACTR3 |
| Gene ID: |
10096 |
| Uniprot ID: |
ARP3_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 47 kDa* doublet corresponding to the molecular mass of Arp3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells. |
| Immunogen: |
Arp3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins. |
| Function | ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis. |
| Protein Name | Actin-Related Protein 3Actin-Like Protein 3 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-3928662Reactome: R-HSA-5663213Reactome: R-HSA-8856828Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionNucleusIn Pre-Apoptotic CellsColocalizes With Mefv In Large Specks (Pyroptosomes) |
| Alternative Antibody Names | Anti-Actin-Related Protein 3 antibodyAnti-Actin-Like Protein 3 antibodyAnti-ACTR3 antibodyAnti-ARP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
