| Host: | Mouse |
| Applications: | FC/ICC/WB |
| Reactivity: | Bovine/Horse/Human/Pig/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Actin for use in FC, ICC and WB in Bovine, Horse, Human, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 5J11 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | IgG |
| Dilution Range: | WB 1:5000-1:10000ICC 1:500-1:1000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
| Specificity: | The antibody reacts with a 42 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry. It reacts across a broad range of mammalian species. |
| Immunogen: | Actin prepared from bovine brain. |
| Immunogen Sequence: | Bovine |
| Background | Our Anti-Actin mouse monoclonal primary antibody detects bovine, horse, human, pig, and rat Actin, and is igg. It is validated for use in FC, ICC, WB. |
Information sourced from Uniprot.org