• Immunofluorescence analysis of L929 cells using ACSS1 Rabbit polyclonal antibody (STJ117203) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ACSS1 in paraffin-embedded Human colon carcinoma using ACSS1 Rabbit polyclonal antibody (STJ117203) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ACSS1 Rabbit polyclonal antibody (STJ117203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACSS1 antibody (340-689) (STJ117203)

SKU:
STJ117203

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSS1 (340-689) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSS1
Gene ID: 84532
Uniprot ID: ACS2L_HUMAN
Immunogen Region: 340-689
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2).
Immunogen Sequence: ITGHSYVVYGPLCNGATSVL FESTPVYPNAGRYWETVERL KINQFYGAPTAVRLLLKYGD AWVKKYDRSSLRTLGSVGEP INCEAWEWLHRVVGDSRCTL VDTWWQTETGGICIAPRPSE EGAEILPAMAMRPFFGIVPV LMDEKGSVVEGSNVSGALCI SQAWPGMARTIYGDHQRFVD AYFKAYPGYYFTGDGAYRTE GGYYQITGRMDDVINISGHR LGTAEIEDAIADHPAVPES
Post Translational Modifications Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions. Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient.
Protein Name Acetyl-Coenzyme A Synthetase 2-Like - Mitochondrial
Acetate--Coa Ligase 2
Acetyl-Coa Synthetase 2
Acecs2
Acyl-Coa Synthetase Short-Chain Family Member 1
Propionate--Coa Ligase
Database Links Reactome: R-HSA-71384
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Acetyl-Coenzyme A Synthetase 2-Like - Mitochondrial antibody
Anti-Acetate--Coa Ligase 2 antibody
Anti-Acetyl-Coa Synthetase 2 antibody
Anti-Acecs2 antibody
Anti-Acyl-Coa Synthetase Short-Chain Family Member 1 antibody
Anti-Propionate--Coa Ligase antibody
Anti-ACSS1 antibody
Anti-ACAS2L antibody
Anti-KIAA1846 antibody

Information sourced from Uniprot.org

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