• Immunoprecipitation analysis of 300ug extracts of Hep G2 cells using 3ug ACSL4 Rabbit polyclonal antibody antibody (STJ28909 1:100). Western blot was performed from the immunoprecipitate using ACSL4 Rabbit polyclonal antibody antibody (STJ28909) at a dilition of 1:2000.
  • Immunofluorescence analysis of NIH/3T3 cells using ACSL4 Rabbit polyclonal antibody (STJ28909) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Hep G2 cells using ACSL4 Rabbit polyclonal antibody (STJ28909) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.

Anti-ACSL4 antibody (42-321) (STJ28909)

SKU:
STJ28909

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSL4 (42-321) is suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSL4
Gene ID: 2182
Uniprot ID: ACSL4_HUMAN
Immunogen Region: 42-321
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1?).
Immunogen Sequence: MAKRIKAKPTSDKPGSPYRS VTHFDSLAVIDIPGADTLDK LFDHAVSKFGKKDSLGTREI LSEENEMQPNGKVFKKLILG NYKWMNYLEVNRRVNNFGSG LTALGLKPKNTIAIFCETRA EWMIAAQTCFKYNFPLVTLY ATLGKEAVVHGLNESEASYL ITSVELLESKLKTALLDISC VKHIIYVDNKAINKAEYPEG FEIHSMQSVEELGSNPENLG IPPSRPTPSDMAIVMYTSG
Function Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
Protein Name Long-Chain-Fatty-Acid--Coa Ligase 4
Arachidonate--Coa Ligase
Long-Chain Acyl-Coa Synthetase 4
Lacs 4
Database Links Reactome: R-HSA-434313
Reactome: R-HSA-75876
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iii Membrane Protein
Peroxisome Membrane
Microsome Membrane
Endoplasmic Reticulum Membrane
Cell Membrane
Alternative Antibody Names Anti-Long-Chain-Fatty-Acid--Coa Ligase 4 antibody
Anti-Arachidonate--Coa Ligase antibody
Anti-Long-Chain Acyl-Coa Synthetase 4 antibody
Anti-Lacs 4 antibody
Anti-ACSL4 antibody
Anti-ACS4 antibody
Anti-FACL4 antibody
Anti-LACS4 antibody

Information sourced from Uniprot.org

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