• Western blot analysis of extracts of various lysates, using ACSL4 antibody (STJ11103299) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 3s.
  • Western blot analysis of extracts of Rat liver, using ACSL4 antibody (STJ11103299) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of mouse liver, using ACSL4 antibody (STJ11103299) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ACSL4 rabbit monoclonal antibody (STJ11103299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using ACSL4 rabbit monoclonal antibody (STJ11103299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using ACSL4 rabbit monoclonal antibody (STJ11103299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ACSL4 rabbit monoclonal antibody (STJ11103299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of HepG2 cells using ACSL4 rabbit monoclonal antibody (STJ11103299, dilution 1:1000) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g ACSL4 antibody (STJ11103299). Western blot was performed from the immunoprecipitate using ACSL4 antibody (STJ11103299) at a dilution of 1:1000.

Anti-ACSL4 antibody (42-321) [S9MR] (STJ11103299)

SKU:
STJ11103299

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:58000-1:232000
IHC-P:1:200-1:2000
IF/ICC:1:200-1:800
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSL4
Gene ID: 2182
Uniprot ID: ACSL4_HUMAN
Immunogen Region: 42-321
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1).
Immunogen Sequence: MAKRIKAKPTSDKPGSPYRS VTHFDSLAVIDIPGADTLDK LFDHAVSKFGKKDSLGTREI LSEENEMQPNGKVFKKLILG NYKWMNYLEVNRRVNNFGSG LTALGLKPKNTIAIFCETRA EWMIAAQTCFKYNFPLVTLY ATLGKEAVVHGLNESEASYL ITSVELLESKLKTALLDISC VKHIIYVDNKAINKAEYPEG FEIHSMQSVEELGSNPENLG IPPSRPTPSDMAIVMYTSG
Function Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
Protein Name Long-Chain-Fatty-Acid--Coa Ligase 4
Arachidonate--Coa Ligase
Long-Chain Acyl-Coa Synthetase 4
Lacs 4
Database Links Reactome: R-HSA-434313
Reactome: R-HSA-75876
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iii Membrane Protein
Peroxisome Membrane
Microsome Membrane
Endoplasmic Reticulum Membrane
Cell Membrane
Alternative Antibody Names Anti-Long-Chain-Fatty-Acid--Coa Ligase 4 antibody
Anti-Arachidonate--Coa Ligase antibody
Anti-Long-Chain Acyl-Coa Synthetase 4 antibody
Anti-Lacs 4 antibody
Anti-ACSL4 antibody
Anti-ACS4 antibody
Anti-FACL4 antibody
Anti-LACS4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance