• Immunohistochemistry analysis of ACSF3 in paraffin-embedded human colon carcinoma tissue using ACSF3 Rabbit polyclonal antibody (STJ11105455) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ACSF3 Rabbit polyclonal antibody (STJ11105455) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.

Anti-ACSF3 antibody (370-576) (STJ11105455)

SKU:
STJ11105455

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Malonate--Coa Ligase Acsf3-Mitochondrial (370-576) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSF3
Gene ID: 197322
Uniprot ID: ACSF3_HUMAN
Immunogen Region: 370-576
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 370-576 of human ACSF3 (NP_777577.2).
Immunogen Sequence: TAVRLPGSVGTPLPGVQVRI VSENPQREACSYTIHAEGDE RGTKVTPGFEEKEGELLVRG PSVFREYWNKPEETKSAFTL DGWFKTGDTVVFKDGQYWIR GRTSVDIIKTGGYKVSALEV EWHLLAHPSITDVAVIGVPD MTWGQRVTAVVTLREGHSLS HRELKEWARNVLAPYAVPSE LVLVEEIPRNQMGKIDKKAL IRHFHPS
Function Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. May have some preference toward very-long-chain substrates.
Protein Name Malonate--Coa Ligase Acsf3 - Mitochondrial
Acyl-Coa Synthetase Family Member 3
Database Links Reactome: R-HSA-75876
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Malonate--Coa Ligase Acsf3 - Mitochondrial antibody
Anti-Acyl-Coa Synthetase Family Member 3 antibody
Anti-ACSF3 antibody
Anti-PSEC0197 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance