| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-ACPP/PAP (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-Recommended concentration, 3-5µg/mlELISA-antibody detection limit dilution 1:64000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
ACP3 |
| Gene ID: |
55 |
| Uniprot ID: |
PPAP_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_001090.2 |
| Specificity: |
This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1). |
| Immunogen Sequence: |
YGIHKQKEKSRLQ |
| Post Translational Modifications | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection). |
| Function | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. |
| Protein Name | Prostatic Acid PhosphatasePap5'-Nucleotidase5'-NtAcid Phosphatase 3Ecto-5'-NucleotidaseProtein Tyrosine Phosphatase Acp3Thiamine MonophosphataseTmpase Cleaved Into - Papf39 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneNucleusCytoplasmCytosolAppears To Shuttle Between The Cell Membrane And Intracellular VesiclesColocalizes With Flot1 At Cell Membrane And In Intracellular VesiclesColocalizes With Lamp2 On The Lysosome Membrane |
| Alternative Antibody Names | Anti-Prostatic Acid Phosphatase antibodyAnti-Pap antibodyAnti-5'-Nucleotidase antibodyAnti-5'-Nt antibodyAnti-Acid Phosphatase 3 antibodyAnti-Ecto-5'-Nucleotidase antibodyAnti-Protein Tyrosine Phosphatase Acp3 antibodyAnti-Thiamine Monophosphatase antibodyAnti-Tmpase Cleaved Into - Papf39 antibodyAnti-ACP3 antibodyAnti-ACPP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
