| Host: |
Mouse |
| Applications: |
IHC/WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Prostatic acid phosphatase (201-300 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ABT119 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2bk |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: |
IHC-P 1:100-500WB 1:200-1000IF 1:100-500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ACP3 |
| Gene ID: |
55 |
| Uniprot ID: |
PPAP_HUMAN |
| Immunogen Region: |
201-300 aa |
| Specificity: |
The antibody can specifically recognize human PSAP protein.. The antibody was also Predict react with Mouse;Rat |
| Immunogen: |
Synthesized peptide derived from the human PSAP at the amino acid range 201-300 |
| Post Translational Modifications | N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection). |
| Function | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Isoform 2: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. PAPf39: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection. |
| Protein Name | Prostatic Acid PhosphatasePap5'-Nucleotidase5'-NtAcid Phosphatase 3Ecto-5'-NucleotidaseProtein Tyrosine Phosphatase Acp3Thiamine MonophosphataseTmpase Cleaved Into - Papf39 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Type I Membrane ProteinLysosome MembraneNucleusCytoplasmCytosolAppears To Shuttle Between The Cell Membrane And Intracellular VesiclesColocalizes With Flot1 At Cell Membrane And In Intracellular VesiclesColocalizes With Lamp2 On The Lysosome Membrane |
| Alternative Antibody Names | Anti-Prostatic Acid Phosphatase antibodyAnti-Pap antibodyAnti-5'-Nucleotidase antibodyAnti-5'-Nt antibodyAnti-Acid Phosphatase 3 antibodyAnti-Ecto-5'-Nucleotidase antibodyAnti-Protein Tyrosine Phosphatase Acp3 antibodyAnti-Thiamine Monophosphatase antibodyAnti-Tmpase Cleaved Into - Papf39 antibodyAnti-ACP3 antibodyAnti-ACPP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit polyclonal antibody (STJ11105635) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/178279/370621/STJ11105635_1__38601.1713136567.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit polyclonal antibody (STJ11105635) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
