• Immunofluorescence analysis of NIH-3T3 cells using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Aconitase 2 (ACO2) in paraffin-embedded rat kidney tissue using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aconitase 2 (ACO2) in paraffin-embedded mouse kidney tissue using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aconitase 2 (ACO2) in paraffin-embedded human colon tissue using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates, using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using Aconitase 2 (ACO2) Rabbit monoclonal antibody (STJ11102095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACO2 antibody (1-150) [S5MR] (STJ11102095)

SKU:
STJ11102095

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:800
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACO2
Gene ID: 50
Uniprot ID: ACON_HUMAN
Immunogen Region: 1-150
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Aconitase 2 (ACO2) (Q99798).
Immunogen Sequence: MAPYSLLVTRLQKALGVRQY HVASVLCQRAKVAMSHFEPN EYIHYDLLEKNINIVRKRLN RPLTLSEKIVYGHLDDPASQ EIERGKSYLRLRPDRVAMQD ATAQMAMLQFISSGLSKVAV PSTIHCDHLIEAQVGGEKDL RRAKDINQEV
Post Translational Modifications Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers.
Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Protein Name Aconitate Hydratase - Mitochondrial
Aconitase
Citrate Hydro-Lyase
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Reactome: R-HSA-9837999
Reactome: R-HSA-9854311
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Aconitate Hydratase - Mitochondrial antibody
Anti-Aconitase antibody
Anti-Citrate Hydro-Lyase antibody
Anti-ACO2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance