• Immunofluorescence analysis of C6 cells using ACHE Rabbit polyclonal antibody (STJ22484) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ACHE Rabbit polyclonal antibody (STJ22484) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ACHE Rabbit polyclonal antibody (STJ22484) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ACHE antibody (STJ22484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACHE antibody (398-617) (STJ22484)

SKU:
STJ22484

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACHE (398-617) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACHE
Gene ID: 43
Uniprot ID: ACES_HUMAN
Immunogen Region: 398-617
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 398-617 of human ACHE (NP_056646.1).
Immunogen Sequence: VPQVSDLAAEAVVLHYTDWL HPEDPARLREALSDVVGDHN VVCPVAQLAGRLAAQGARVY AYVFEHRASTLSWPLWMGVP HGYEIEFIFGIPLDPSRNYT AEEKIFAQRLMRYWANFART GDPNEPRDPKAPQWPPYTAG AQQYVSLDLRPLEVRRGLRA QACAFWNRFLPKLLSATASE APSTCPGFTHGEAAPRPGLP LPLLLLHQLLLLFLSHLRRL
Tissue Specificity Isoform H is highly expressed in erythrocytes.
Function Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Protein Name Acetylcholinesterase
Ache
Database Links Reactome: R-HSA-112311
Reactome: R-HSA-1483191
Reactome: R-HSA-422085
Cellular Localisation Synapse
Secreted
Cell Membrane
Peripheral Membrane Protein
Isoform T: Nucleus
Only Observed In Apoptotic Nuclei
Isoform H: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative Antibody Names Anti-Acetylcholinesterase antibody
Anti-Ache antibody
Anti-ACHE antibody

Information sourced from Uniprot.org

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