| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Acetyl-Sex-determining region Y protein-Lys136 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SRY |
| Gene ID: |
6736 |
| Uniprot ID: |
SRY_HUMAN |
| Specificity: |
This antibody detects endogenous levels of Human SRY (Acetyl Lys136) |
| Immunogen: |
Synthesized peptide derived from human SRY (Acetyl Lys136) |
| Post Translational Modifications | Phosphorylated on serine residues by PKA. Phosphorylation by PKA enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-136 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylated by PARP1. ADP-ribosylation reduces its DNA-binding activity. |
| Function | Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Involved in different aspects of gene regulation including promoter activation or repression. Binds to the DNA consensus sequence 5'-ATAACAAAT-3'. SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending. Also involved in pre-mRNA splicing. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. |
| Protein Name | Sex-Determining Region Y ProteinTestis-Determining Factor |
| Database Links | Reactome: R-HSA-3769402Reactome: R-HSA-9690406 |
| Cellular Localisation | Nucleus SpeckleCytoplasmNucleusAcetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic DelocalizationColocalizes With Sox6 In SpecklesColocalizes With Caml In The NucleusColocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)Nuclear Import Is Facilitated By Xpo4A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Sex-Determining Region Y Protein antibodyAnti-Testis-Determining Factor antibodyAnti-SRY antibodyAnti-TDF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
