• Western blot analysis of HeLa, using Acetyl-Histone H3-K9 rabbit polyclonal antibody (STJ29397) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of NIH/3T3, using Acetyl-Histone H3-K9 rabbit polyclonal antibody (STJ29397) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of C6, using Acetyl-Histone H3-K9 rabbit polyclonal antibody (STJ29397) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours (left). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (STJ29397) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours (left). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 antibody (STJ29397) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Acetyl-Histone H3-K9 antibody (2-19) (STJ29397)

SKU:
STJ29397

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H3-K9 (2-19) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 2-19
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-19 of human Histone H3 (NP_003520.1).
Immunogen Sequence: ARTKQTARKSTGGKAPRK
Background The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9).

Information sourced from Uniprot.org

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