• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody (STJ118518) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at 1:400 dilution. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.
  • Western blot analysis of lysates from C6 cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at 1:400 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H3-K4 Rabbit polyclonal antibody (STJ118518) at 1:400 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 60s.

Anti-Acetyl-Histone H3-K4 antibody (STJ118518)

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STJ118518

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H3-K4 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:100
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1).
Immunogen Sequence: ARTKQ
Background This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer s disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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