• Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (STJ11103508) at 1:1000 dilution. HeLa NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K27 antibody (STJ11103508) at 1:1000 dilution.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Acetyl-Histone H3-K27 rabbit monoclonal antibody (STJ11103508) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Acetyl-Histone H3-K27 rabbit monoclonal antibody (STJ11103508) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 rabbit monoclonal antibody (STJ11103508) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Acetyl-Histone H3-K27 rabbit monoclonal antibody (STJ11103508) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa treated with TSA and HeLa cells using Acetyl-Histone H3-K27 rabbit monoclonal antibody (STJ11103508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of Hela TSA cells using 5 Mu g Acetyl-Histone H3-K27 antibody (STJ11103508). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K27 antibody (STJ11103508) at a dilution of 1:50000.
  • CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105 K562 cells with 2 ug Acetyl-Histone H3-K27 rabbit monoclonal antibody, along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Acetyl-Histone H3-K27 in representative gene loci (RPL30) , as shown in figure.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (STJ11103508) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Anti-Acetyl-Histone H3-K23 antibody [S8MR] (STJ11103508)

Anti-Acetyl-Histone H3-K23 antibody [S8MR] (STJ11103508)

SKU:
STJ11103508

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC/IP/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Acetyl-Histone H3-K23 is suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
CUT&Tag 10⁵ cells/2 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K27 of human Histone H3 (P68431).
Immunogen Sequence: MARTKQTARKSTGGKAPRKQ LATKAARKSAPATGGVKKPH RYRPGTVALREIRRYQKSTE LLIRKLPFQRLVREIAQDFK TDLRFQSSAVMALQEACEAY
Background Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Information sourced from Uniprot.org

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