• Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (STJ29399) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (STJ29399) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody (STJ29399) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (STJ29399) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (STJ29399) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (STJ29399) at dilution of 1:100 (40x lens).
  • Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (STJ29399) at 1:1000 dilution.
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (STJ29399) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-Acetyl-Histone H3-K18 antibody (STJ29399)

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STJ29399
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Host: Rabbit
Applications: WB, IHC, IF, IP, ChIP, ChIPseq
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:1000-1:3000
IHC: 1:200-1:500
IF: 1:500-1:1000
IP: 1:200-1:500
ChIP: 1:50-1:100
ChIPseq: 1:50-1:100
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
1 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Information sourced from uniprot.org

6 months standard