• Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Acetyl-Histone H2B-K5 Rabbit polyclonal antibody (STJ118069) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Acetyl-Histone H2B-K5 antibody (STJ118069)

SKU:
STJ118069

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H2B-K5 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K5 of human Histone H2B (NP_003519.1).
Immunogen Sequence: PAKSA
Background This gene encodes the pulmonary-associated surfactant protein B (SPB) , an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.

Information sourced from Uniprot.org

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