Anti-Acetyl-DNMT1-K1127/K1129/K1131/K1133 antibody (STJ27564)

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STJ27564
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Host: Rabbit
Applications: IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against DNMT1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: IHC: 1:50-1:100
IF: 1:50-1:100
Uniprot ID: DNMT1_HUMAN
Gene ID: 1786
Gene Symbol: DNMT1
Immunogen: A synthetic peptide of human DNMT1
Background This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Tissue Specificity Ubiquitous.highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Post Translational Modifications Sumoylated.sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation. Ubiquitinated by UHRF1.interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
Database Links Reactome: R-HSA-212300
Cellular Localisation Nucleus
Protein Name Dna
Cytosine-5-Methyltransferase 1
Dnmt1
Cxxc-Type Zinc Finger Protein 9
Dna Methyltransferase Hsai
Dna Mtase Hsai
M.hsai
Mcmt
Alternative Names Anti-Dna antibody
Anti-Cytosine-5-Methyltransferase 1 antibody
Anti-Dnmt1 antibody
Anti-Cxxc-Type Zinc Finger Protein 9 antibody
Anti-Dna Methyltransferase Hsai antibody
Anti-Dna Mtase Hsai antibody
Anti-M.hsai antibody
Anti-Mcmt antibody
Anti-DNMT1 antibody
Anti-AIM antibody
Anti-CXXC9 antibody
Anti-DNMT antibody

Information sourced from uniprot.org

6 months standard