Anti-Acetyl-Coenzyme A Carboxylase antibody [R07-2G8] (STJA0033974)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Acetyl Coenzyme A Carboxylase
General Information
| Short Description | Rabbit monoclonal anti-Acetyl-Coenzyme A Carboxylase for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-2G8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ACACB |
| Gene ID | 32 |
| Uniprot ID | ACACB_HUMAN |
| Immunogen | A synthetic peptide of human Acetyl Coenzyme A Carboxylase |
Additional Info
| Tissue Specificity | Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon. Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis. |
| Post Translational Modifications | The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity. Phosphorylation at Ser-222 by AMPK inactivates the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis. |
| Function | Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation. Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage. |
| Protein Name | Acetyl-Coa Carboxylase 2Acc-Beta |
| Database Links | Reactome: R-HSA-163765Reactome: R-HSA-196780Reactome: R-HSA-200425Reactome: R-HSA-2426168 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Acetyl-Coa Carboxylase 2 antibodyAnti-Acc-Beta antibodyAnti-ACACB antibodyAnti-ACC2 antibodyAnti-ACCB antibody |
Information sourced from Uniprot.org