• Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody.
  • Western blot analysis of A549 cells using ACAT-1 Polyclonal Antibody
  • Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using ACAT-1 Polyclonal Antibody

Anti-ACAT1 antibody (221-270 aa) (STJ91436)

SKU:
STJ91436

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-CoA acetyltransferase, mitochondrial (221-270 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAT1
Gene ID: 38
Uniprot ID: THIL_HUMAN
Immunogen Region: 221-270 aa
Specificity: ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ACAT1 at the amino acid range 221-270
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Protein Name Acetyl-Coa Acetyltransferase - Mitochondrial
Acetoacetyl-Coa Thiolase
T2
Database Links Reactome: R-HSA-70895
Reactome: R-HSA-77108
Reactome: R-HSA-77111
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Acetyl-Coa Acetyltransferase - Mitochondrial antibody
Anti-Acetoacetyl-Coa Thiolase antibody
Anti-T2 antibody
Anti-ACAT1 antibody
Anti-ACAT antibody
Anti-MAT antibody

Information sourced from Uniprot.org

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